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1. Define restriction enzymes, and outline how they are used to make recombinant DNA.

2. Outline the PCR process and provide an example of its use.

3. Describe five ways of getting DNA into a cell.

4. Describe how a gene library is made.

5. Differentiate between cDNA and synthetic DNA.

6. Diagram the Southern blot procedure and provide an example of its use.

7. Discuss the advantages of the three-domain system.

8. List the characteristics of the Bacteria, Archaea, and Eukarya domains.

9. Differentiate among eukaryotic, prokaryotic, and viral species.

10. List the major characteristics used to differentiate the three kingdoms of multicellular Eukarya.

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