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You perform column chromatography on a mixture of reaction products which shows two spots on TLC. However, under the column conditions you chose those two compounds do not separate. a. One can alter a number of factors in order to improve the resolute of components by column chromatography. For each of the factors listed in the following table, separation can be improved by either increasing or decreasing the factor. For each, describe the changes in sample size or interactions between components and the chromatography medium are altered by increasing or decreasing the factor so that the resolution will be improved. 1. Column Diameter: 2. Column Length: 3. Sample Volume: 4. Sample Concentration: 5. Loading Time: 6. Flow Rate:

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