You need to make 500 ml of 100 mm potassium phosphate, Ask an Expert

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You need to make 500 ml of 100 mM potassium phosphate buffer at pH 7.5. You have the stock solutions of KH2PO4 and K2HPO4 at 1M each. You will make the buffer by adding some of each of these stock solutions to water. How many mls of the KH2PO4 stock solution will you use? The pKa values for phosphate are 2.12, 7.21, and 12.3.

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