You have a 25kd histidine tagged bacterial protein 100 pure, Ask an Expert

Academics

You have a 25kD histidine tagged bacterial protein (100% pure). You apply this pure protein to a Ni+2 agarose column. Then you apply a crude mouse cell extract to the column. After extensive washing, you apply an imidazole buffer. Analysis of the resultant elution on an SDS-PAGE gel indicates two proteins (25kD and 75kD) of equal coomassie blue staining. What is the simplest explanation for why the 25kD and the 75kD proteins copurified off the column?

View complete question

Chemistry, Academics

Category:- Chemistry
Reference No.:- M91021658

Have any Question?Write your Review or question?

Related Questions in Chemistry

A student takes 50ml of a stock fescn2 solution and dilutes

A student takes 5.0mL of a stock FeSCN2+ solution and dilutes it with deionized water to a volume of 50mL. He then puts the solution in a Spectronic 20 instrument and determines the the % transmittance of the new solutio ...

What is an example of a specific transition metal solution

What is an example of a specific transition metal solution, including the oxidation state of the transition metal, and the color of the solution, and the complex ion that the transition metal forms in that solution if ap ...

For each of the following reactions identify the oxidizing

For each of the following reactions identify the oxidizing agent and the reducing agent. 2Na(aq)+2H2O(l)→2NaOH(aq)+H2(g) C(s)+O2(g)→CO2(g) 2MnO-4(aq)+5SO2(g)+2H2O(l)→2Mn2+(aq)+5SO2-4(aq)+4H+(aq)

If liquid is at or above its flash point the flame spread

If liquid is at or above it's flash point, the flame spread rate is fast, and the entire pool is engulfed within seconds. In such a case, the liquid is already evaporating sufficiently to reach the lower flammability lim ...

A chemist adds 3700 ml of a 0371m barium acetate bac2h3o22

A chemist adds 370.0 mL of a 0.371M barium acetate (Ba(C2H3O2)2) solution to a reaction flask. Calculate the millimoles of barium acetate the chemist has added to the flask. Round your answer to 3 significant digits.

How many formula units ofnbspna2so4are present in

How many formula units of Na2SO4are present in a 450 gram sample of the salt? Use 6.022×1023mol-1 or Avogadro's number. Report your answer in scientific notation. Your answer should have two significant figures.

How many grams of h2so4 must be dissolved into 0709 l of

How many grams of H2SO4 must be dissolved into 0.709 L of solution to generate a concentration of 0.303 M?

A paint contains 1800 ppm lead approximately what mass of

A paint contains 180.0 ppm lead. Approximately what mass of lead will be in 1.00 cm ^3 of this paint when 1.00 gal is uniformly applied to 445.0 ft 2 of a wall. The density of the paint is 8.00 lb/gal.

A 200 ml sample of 0200 m hbr solution is titratedwith 0200

A 20.0 mL sample of 0.200 M HBr solution is titratedwith 0.200 M NaOH solution. Calculate the pH of the solution after the following volumes of base have been added. (19.8 mL)

Label each of the following phase changes as either

Label each of the following phase changes as either exothermic or endothermic. i. H 2 O( s ) + heat H 2 O( l ) (1 point) ii. H 2 O( g ) H 2 O( l ) + heat (1 point)

4,153,160 Questions Asked
13,132 Experts
2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Recent Questions

Ask Chemistry Expert