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You are given a stock solution of EDTA at a concentration of 5mM, how would you dilution of EDTA with a final concentration of 5µM in a final volume of 250mL?  You need to prepare a stock solution of medium for your culture cells, which usually includes liquid salt solution and bovine serum. Your liquid salt solution is supplied in a 10X concentration, and you need to dilute it to 1X for use. You also need to add 100% fetal bovine serum for a final concentration of 10%. How would you make up 5 liters of this culture media using water as your solvent?

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