Why will fluorescein elute from an alumina-packed column, Ask an Expert

Academics

Why will fluorescein elute from an alumina-packed column when NaOH is added as a solvent? Why will methylene blue not elute using NaOH as a solvent?

- Is it because fluorescein is polar, therefore it will be more soluble in the NaOH? Or is it that it is slightly acidic and adding NaOH will create Al-OH groups in the alumina, causing the fluorescein to adsorb better to the stationary phase instead of the mobile phase?

View complete question

Chemistry, Academics

Category:- Chemistry
Reference No.:- M91020845

Have any Question?Write your Review or question?

Related Questions in Chemistry

An electron is ejected from a metal with a velocity 778

An electron is ejected from a metal with a velocity 7.78 x10^5 m/s. If the metal has a threshold frequency of 9.90 x1014 Hz, what was the wavelength of the incident photon?

Determine if the reactants shown on the right would result

Determine if the reactants shown on the right would result in the formation of a precipitate. Choices can be used more than one time or not at all. Forms a precipitate Will not form a precipitate Na 2 SO 4 + Ba(NO 3 ) 2 ...

Interpretation of reactions by ionic type equations aqueous

Interpretation of Reactions by Ionic Type Equations. Aqueous solutions of the following substances or their mixtures with water if they are only slightly soluble, are mixed. Write first the conventional equation , secon ...

Ironiii oxide reacts with carbon monoxide according to the

Iron(III) oxide reacts with carbon monoxide according to the equation: Fe2O3(s)+3CO(g)→2Fe(s)+3CO2(g) A reaction mixture initially contains 22.15 g Fe2O3 and 15.98 g CO. Once the reaction has occurred as completely as po ...

If 1440 grams of rbcl are dissolved in water to make a

If 14.40 grams of RbCl are dissolved in water to make a solution of 0.224 L, the density is found to be 1.047 g/cm 3 . Calculate the molality of the solute.

A 200 ml sample of 0200 m hbr solution is titratedwith 0200

A 20.0 mL sample of 0.200 M HBr solution is titratedwith 0.200 M NaOH solution. Calculate the pH of the solution after the following volumes of base have been added. (19.8 mL)

A student is doing the experiment chemical formulas of

A student is doing the experiment Chemical Formulas of Copper Sulfide by heating copper with excess of sulfur at very high temperatures using a Bunsen burner. Copper and sulfur react stoichiometrically and excess of sulf ...

How many formula units ofnbspna2so4are present in

How many formula units of Na2SO4are present in a 450 gram sample of the salt? Use 6.022×1023mol-1 or Avogadro's number. Report your answer in scientific notation. Your answer should have two significant figures.

0394 mol of a solid was dissolved in 340 ml of water at 269

0.394 mol of a solid was dissolved in 340 mL of water at 26.9 oC. After the solid had fully dissolved, the final temperature of the solution was 31.6 oC. What is the molar heat of solution of the substance?

How many grams of h2so4 must be dissolved into 0709 l of

How many grams of H2SO4 must be dissolved into 0.709 L of solution to generate a concentration of 0.303 M?

4,153,160 Questions Asked
13,132 Experts
2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Recent Questions

Ask Chemistry Expert