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While separating the components of a mixture of Blue Dextran, cytochrome c, as well as DNP-glycine by ion exchange chromatography, the eluent is changed from 0.1 M acetate buffer to 1.0 M acetate buffer once the first colored component begins to exit the column. Explain why is the eluent changed from low salt concentration to high salt concentration? Define the order in which the components would elute from the CM Sephadex column.

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Category:- Chemistry
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