(Answered) When the base pair at in the middle of a short 20

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When the base pair A=T in the middle of a short (20 nucleotide) synthetic duplex DNA strand was replaced with a. Z F base pair (shown below) the duplex was destabilized by about 13.4 kJ/mol.

However, when the pair was at the end of the strand it was in fact more stabilizing than A=T.

Explain these results in light of weak forces responsible for the DNA double helix.

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