An enzyme-catalyzed reaction was carried out in a 0.1 M Tris buffer, pH 7.8. As a result of the reaction, 0.015 mole/Liter of H+ was produced. Note that the pKa of Tris is 8.1.

a) What was the ratio of Tris+ (protonated form)/Tris0 (conjugate base) at the start of the reaction?

b) What were the concentrations of Tris+ and Tris0 at the start of the reaction?

c) What were the concentrations of Tris0 and Tris+ at the end of the reaction?

d) What was the pH at the end of the reaction?

e) What would the final pH be in the absence of any buffer?