Lysozyme was assayed at bacterial cell wall, [S] = 2.5x10-8 g. The Km for the substrate = 6x10-3g. At the end of one minute 2.5% of the cell wall hydrolysed. Answer the following questions using this information:
a) What percent of bacetrial cell wall will be hydrolyzed at the end of 3 minutes assuming product formation is in 1st order of reaction?
b) What will be a concentration of hydrolyzed product at the end of 4 minutes?
c) Determine maximal velocity (Vmax) with the enzyme concentration used.