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A biochemical reaction is to be carried out in 250.00 mL solution and buffered to physiologic pH of 7.20 using a conjugate acid/base pair, pKa = 6.98. Because of the fragility of the proteins involved and their limited solubility, the pH must not change by more than + 0.05 pH units during the reaction. What must the concentration be, of each member of the buffering acid/base pair, if the reaction is expected to release 0.1 millimoles of H+?

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