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An enzyme (MW = 20,25) catalyzes the hydrolysis of a newly discovered substrate. The kcat was found to be 3500 1/sec and Km = 0.05M. What is the Vmax of this enzyme when enzyme concentration is equal to 0.2mg/mL? I understand the equation to find kcat is: kcat = Vmax/[E]t however, [E]t needs to be in molarity. It sounds simple, but I am not getting how to go from 0.2mg/mL to moles/L.

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