Two chromatographic columns gc are being compared for use, Ask an Expert

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Question 1: Two chromatographic columns (GC) are being compared for use in an analysis. One is a 50 cm long packed column that is 5 mm in inside diameter (ID), with a stationary phase that is 1um thick on the support packing and has a total of 25,000 theoretical plates. The other column is 1 meter long, also 5 mm ID, with the same thickness of stationary phase on the support, and has 40,000 theoretical plates. Ignoring any other variables:

a) Which column would provide the best separation of sample peaks? Why?
b) Which column would provide the sharpest peaks? Why?
c)If the 50 cm column had a stationary phase thickness of 2 um on the support, how would that affect the results? (Hint-"C" factor of Van Deemter Eq.)

Question 2: The use of an FTIR Transducer has never become commonly used as a detector for HPLC because?

Question 3: In High Resolution Carbon NMR Spectroscopy, the phenomenon of "Spin-Spin Coupling" is rarely seen and, if seen, is very minor. Very briefly, why is this true?

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Category:- Chemistry
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