Ask Question, Ask an Expert

+61-413 786 465

info@mywordsolution.com

Ask Chemistry Expert

The Sanger dideoxy method for determining DNA sequence is limited in that a stretch of only 1000 or fewer bases can be analyzed in one reaction. Suppose you wish to sequence a newly isolated double-strand DNA tumor virus that contains 5000 base pairs. You decide to use the Sanger method on restriction fragments of the DNA for sequencing. You use an enzyme that makes a significant number of cuts to give, on average, fragments of 300 nucleotides or less.

Why might it be a good idea also to sequence a second set of fragments cleaved by another restriction enzyme?

Chemistry, Academics

  • Category:- Chemistry
  • Reference No.:- M91885296
  • Price:- $10

Priced at Now at $10, Verified Solution

Have any Question?


Related Questions in Chemistry

For the equilibrium2ibrgi2gbr2gnbspkp85times10-3 at 150

For the equilibrium 2IBr( g )?I2( g )+Br2( g )  Kp =8.5×10-3 at 150 °C. Part A: If 2.7×10-2 atm of IBr is placed in a 2.0-L container, what is the partial pressure of IBr after equilibrium is reached? Express your answer ...

It takes 378 ml of 02114 m naoh to neutralize 193 ml of an

It takes 37.8 mL of 0.2114 M NaOH to neutralize 19.3 mL of an oxalic acid solution with an unknown concentration. Calculate the actual concentration of this oxalic acid solution. Don't forget to look at the balanced equa ...

1 potassium hydrogen phthalate is a solid monoprotic acid

1. Potassium hydrogen phthalate is a solid, monoprotic acid frequently used in the laboratory as a primary standard. It has the unwieldy formula of  KHC 8 H 4 O 4 . This is often written in shorthand notation as KHP. If  ...

You will have a 500ml solution of salt water from this you

You will have a 500mL solution of salt water. From this you will take 10.0mL and place it on a balance. You find that the 10.0mL solution has a mass of 12.5 g. What is the density of the 500mL of salt water solution in g ...

Consider the following reactionco h2 ltgt ch3oha reaction

Consider the following reaction: CO + H2 CH3OH A reaction mixture in a 5.18 L flask at a certain temperature contains 26.8 g CO and 2.36 g H2 . At equilibrium, the flask contains 8.67 g CH3OH. Calculate the equilibrium c ...

What is the calculation of kcnbspfor the following reaction

What is the calculation of K c  for the following reaction if an initial reaction mixture of 0.600 mole of CO and 1.80 mole of H 2  in a 6.00 liter container forms an equilibrium mixture containing 0.226 mole of H 2 O an ...

Determine the molecular formula of a compound that contains

Determine the molecular formula of a compound that contains 26.7% P, 12.1% N, and 61.2% Cl, and has a molar mass of 580.0 g/mol. Enter chemical formula as is HC2H3O2 as HC2H3O2.

Explain why the mass of the crucible and anhydrous salt is

Explain why the mass of the crucible and anhydrous salt is less than the mass of the crucible and the hydrate. What is the chemical formula of copper (II) sulfate?

202 g of an unknown molecular solute is dissolved in 565

20.2 g of an unknown molecular solute is dissolved in 5.65 moles of aniline (C6H5NH2), causing the boiling point of aniline to increase by 3.79 °C. Calculate the molar mass of the solute. For aniline, the molar mass is 9 ...

Suppose 1000 ml of an aqueous solution containing an

Suppose 100.0 mL of an aqueous solution containing an unknown monoprotic acid (called HA) is titrated with 0.150 M KOH. The titration requires 47.82 mL of the potassium hydroxide to reach the equivalence point. What is t ...

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Why might a bank avoid the use of interest rate swaps even

Why might a bank avoid the use of interest rate swaps, even when the institution is exposed to significant interest rate

Describe the difference between zero coupon bonds and

Describe the difference between zero coupon bonds and coupon bonds. Under what conditions will a coupon bond sell at a p

Compute the present value of an annuity of 880 per year

Compute the present value of an annuity of $ 880 per year for 16 years, given a discount rate of 6 percent per annum. As

Compute the present value of an 1150 payment made in ten

Compute the present value of an $1,150 payment made in ten years when the discount rate is 12 percent. (Do not round int

Compute the present value of an annuity of 699 per year

Compute the present value of an annuity of $ 699 per year for 19 years, given a discount rate of 6 percent per annum. As