The protein needs to be in 1x buffer and in a total volume, Ask an Expert

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You want to load 15ug of protein in 10uL into one of the 12% polyacrylamide gel wells. The protein needs to be in 1X buffer and in a total volume of 0.100 mL. You are given a 5mg/mL protein solution, a 20X sample buffer, and distilled water. How much of each wuld you mix together to make the required volume?

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