The protein lysozyme unfolds at a transition temperature of 75.5°C and the standard enthalpy of transition is 509 kJ mol-1. Calculate the entropy of unfolding of lysozyme at 25.0°C, given that the difference in the constant-pressure heat capacities upon unfolding is 6.28 kJ K-1mol-1 and can be assumed to be independent of temperature. Hint. Imagine that the transition at 25.0°C occurs in three steps: (i) heating of the folded protein from 25.0°C to the transition temperature, (ii) unfolding at the transition temperature, and (iii) cooling of the unfolded protein to 25.0°C. Because the entropy is a state function, the entropy change at 25.0°C is equal to the sum of the entropy changes of the steps.