The peak absorbance of the first chromatographic peak at, Ask an Expert

Academics

A mixture of A and B was partially separated on an HPLC column, resultin in overlap of their chromatographic peaks. The absorbance of the effluent was monitored at 300nm (?A = 1800, ?B= 1600) and 350 nm (?A2100 and ?B=3200). The peak absorbance of the first chromatographic peak at 300nm was 0.020 and at 350nm 0.030. What is the main component of the first eluting peak and what percentage of the total absorbance at 300nm is due to this component?(assuming the light path- length = 1.0 cm)

View complete question

Chemistry, Academics

Category:- Chemistry
Reference No.:- M9472335

Have any Question?Write your Review or question?

Related Questions in Chemistry

H2g i2g 2hitemperature 731 kif 160 mol h2nbspand 420 mol

H 2 (g) + I 2 (g) = 2HI Temperature = 731 K If 1.60 mol H 2 and 4.20 mol I 2 areplaced in a 1.08 L vessel What is the equilibrium concentration of H 2 in the gaseous mixture? The equilibrium constant is K = 49.7

You will have a 500ml solution of salt water from this you

You will have a 500mL solution of salt water. From this you will take 10.0mL and place it on a balance. You find that the 10.0mL solution has a mass of 12.5 g. What is the density of the 500mL of salt water solution in g ...

What is the calculation of the new temperature of a gas

What is the calculation of the new temperature of a gas sample has an original volume of 740 ml when collected at 710 mm and 35 o C when the volume becomes 460 ml at 1.20 atm?

2k3po4aq3mgcl2aq rarr mg3po42s6kclaqthe molecular equation

2K3PO4(aq)+3MgCl2(aq) → Mg3(PO4)2(s)+6KCl(aq) The molecular equation you determined in Part B is shown above for your convenience. Examine each of the chemical species involved to determine the ions that would be present ...

A 315-nbspsample ofnbspnh4no3nbspis introduced intoan

A 3.15- sample of NH4NO3 is introduced intoan evacuated 2.26- L flask and then heated to 245 C. What is the total gas pressure, in atmospheres, in the flask at245 C when the NH4NO3 has completely decomposed?

1 potassium hydrogen phthalate is a solid monoprotic acid

1. Potassium hydrogen phthalate is a solid, monoprotic acid frequently used in the laboratory as a primary standard. It has the unwieldy formula of KHC 8 H 4 O 4 . This is often written in shorthand notation as KHP. If ...

A sample of a substance containing only c h and n is burned

A sample of a substance (containing only C, H, and N) is burned in oxygen. 8.696 g of CO 2 , 2.225 g of H 2 O and 4.446 g of NO are the sole products of combustion. What is the empirical formula of the compou ...

Consider the following reactioncog cl2nbspg

Consider the following reaction. CO(g) + Cl 2 (g) > COCl 2 (g) The mechanism is believed to be, (1) Cl 2 2Cl (fast equilibrium) (2) Cl + CO COCl (fast equilibrium) (3) COCl + Cl 2 > COCl 2 + Cl (slow) (4) ...

What is the minimum amount of 60 m h2so4nbspnecessary to

What is the minimum amount of 6.0 M H 2 SO 4 necessary to produce 25.0g of H 2 (g) according to the reaction between aluminum and sulfuric acid? 2 Al(s) + 3 H 2 SO 4 (aq) --------> Al 2 (SO 4 ) 3 (aq) + 3 H 2 (g)

At a given pressure and temperature it takes 1987 sec for a

At a given pressure and temperature, it takes 198.7 sec for a 2.6 L sample of Kr to effuse through a porous membrane. How long does it take for 2.6 L Ar to effuse under the same conditions?

4,153,160 Questions Asked
13,132 Experts
2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Recent Questions

Ask Chemistry Expert