The most accurate values of absorbance are obtained in the range of A = 0.3-2. Which of the following reasons account for lowered accuracy at higher or lower absorbancy values? (Check all that apply).
1) Too little light reaches the detector if absorbance is very high.
2) Beer's law is not followed at very high absorbance values.
3) Too little light reaches the detector if absorbance is very low.
4) Too little difference between sample and reference cell if absorbance is very high.
5) Beer's law is not followed at very low absorbance values.
6) Too little difference between sample and reference cell if absorbance is very low.