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The Keq for imidazole binding to hemoglobin can be determined from the following linear equation: 1/?A100Keq * 1/[lm +1/?A100=(1/?A) a. For the plot of the reciprocal of the change in absorbance versus the reciprocal of the imidazole concentration, identify the equivalent of the slope and the y-intercept in the above equation. Copy the equation in your notebook, and clearly mark a circle around the term equivalent to slope and a square around the y-intercept. b. If the best-fit line determined for a set of data gives a slope of 0.072 M and an intercept of 15. What is the value of Keq and ?A100? c. For the reaction, Hb•Fe+(aq) + Im(aq) ? Hb•Fe-Im+(aq), what does the calculated value of Keq from question 3b predict for the favorability of reactants or products?

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Category:- Chemistry
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