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The denaturation of double stranded DNA to 2 single strands of DNA (dsDNA ? 2 ssDNA) is described by the following T dependence of the equilibrium constant Keq = [ssDNA]2/[dsDNA]. Calculate for the process ?G°, ?H°, ?S°, and DCp at 36.8oC (the ‘°' refers to the reference temperature of 36.8°C). Attach all work and a well labeled van't Hoff plot and explain any assumptions you make. Finally, calculate ?G at 298K. ?T(°C)? K (M)? ? 31.8? 5.56 x 104? ? 36.8? 1.39 x 105? ? 41.8? 4.33 x 105? ? 46.7? 1.44 x 106 Summary of Results: ??G° = ?H° = ?S° = DCp = ?G at 298K = 1

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