The compound malonate is an analog of succinate and is a strong inhibitor of succinate dehydrogenase. The active site of the enzyme will bind to either molecule, however malonate will not undergo any reaction and gets stuck on the enzyme.

Having this in mind answer the following questions with explanation

A) How many NADH's will be produced from the introduction of a single pyruvate molecule to the mitochondrion that is poisoned with malonate (assume that there are adequate amounts of all needed substances)?

B) How many FADH2's will be produced from the introduction of a single pyruvate molecule to the mitochondrion that is poisoned with malonate (assume that there are adequate amounts of all needed substances)?

C) How many ATP's will be produced from the introduction of a single pyruvate molecule to the mitochondrion that is poisoned with malonate (assume that there are adequate amounts of all needed substances)?

2. Assume that you have a normal mitochondrion in a normal cell (there are no inhibitors present). The amino acid glutamate can be converted into alpha ketoglutarate which then can enter the Citric Acid Cycle.

A) If glutamate is the only metabolite present (no acetyl CoA is present) , what intermediate of the cycle will accumulate as glutamate enters the Krebs Cycle or where will it stop? Explain.

B) How many NADH's will be produced by the metabolism of a single molecule of glutamate in the Citric Acid Cycle?

C) How many FADH2's will be produced by the metabolism of a single molecule of glutamate in the Citric Acid Cycle?

D) How many ATP's will be produced by the metabolism of a single molecule of glutamate in the Citric Acid Cycle?

E) How many carbon dioxide molecules will be produced by the metabolism of a single molecule of glutamate in the Citric Acid Cycle?

F) How many oxygen molecules are normally used during the Citric Acid Cycle?