eate a ratio that compares how long it took for a catalyzed reaction vs. non-catalyzed reaction to produce a signal. The catalyzed reaction took 2 minutes. The non-catalyzed reaction took 30. Solutions in each consisted of 5 mL phosphate citrate buffer, 0.1 mL 1% peroxide solution and 0.2 mL TMB solution. The catalyzed reaction used peroxidase.