The amino acid l-histidine has three acid-base equilibria, Ask an Expert

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The amino acid L-histidine has three acid-base equilibria, with pKa values of 1.78, 5.97, and 8.97.

(a) Plot the fraction of solute species present in solution in the pH range 0 to 14.

Use the graph to determine

(b) The major species present in solution at pH = 7.5.

(d) The pH necessary to achieve greater than 99% fully deprotonated histidine.

(e) The pH range for which three different deprotonated forms are present in significant quantities.

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