Suppose the a595 of your unknown was gt 2000 so you had to, Ask an Expert

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Suppose the A595 of your unknown was > 2.000 so you had to dilute your unknown (it was too concentrated). You initially diluted the unknown 1:2 but it was still off the graph. You diluted the 1:2 dilution 1:3 and finally got a reading that fit onto your standard curve. Your absorbance was 0.450. The best fit line equation : y=0.3413x. What is the actual concentration your unknown protein?

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