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Sulfhydryl groups comprise the ability to react with the alkylating reagent N-ethylmaleimide (NEM) in chemical modification experiments. While NEM is added to a purified solution of creatine kinase, Cys 278 is alkylated, however no other Cys residues in the protein are modified. Explain what can infer about the Cys 278 residue based on this observation?

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