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Saliva pH is usually around 6.2-7.4 ... You decide to use a phosphate buffer at 50mM to test activity of a protein. Since phosphate ionization of 2.14, 6.86 and 12.4. you make stock buffer 500mM in 1L. You decide to make one stock pH 6.5 and the other ph 7.2 How many mmols of what type of phosphate componet do you add to each of these buffers?

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