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PROTEIN FOLDING ASSIGNMENT

In an "in vitro" protein folding experiment monitored by fluorescence measurements the following data was obtained for protein X:

[GuHCl], M	Wavelength Maxima, nm
0	330
0.5	332
1.0	331
1.5	332
2.0	336
2.5	341
3.0	348
3.5	355
4.0	357
4.5	359
5.0	358
5.5	359
6.0	360

Use the above data to calculate the fraction of protein unfolded at each denaturant concentration. Plot the results to obtain a protein denaturation profile and calculate the transition mid-point of unfolding. What is the significance of the transition curve? What does the mid-point signify? What protein folding process does the data imply?

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