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Problem- Two isotopic compounds are produced in the synthesis of a new therapeutic drug which must be separated by repeated chromatography cycles. In each cycle, the solution containing both compounds must pass through a chromatography column with a length L = 60.0 cm. The column has N theoretical plates and the unadjusted relative retention is? = 1.001. Calculate the number of theoretical plates N in the column of length L. The observed resolution is 1.81 after 18 cycles?

Now, calculate the plate height in the column in micrometers?

Estimate the resolution in 2 cycles.

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Category:- Chemistry
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