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Problem- 100 mL of a 275 mM sodium phosphate buffer containing 2.75 mM MgCl2 at pH 7.5 will be prepared. The ff. will be available: phosphoric acid(15M), monosodium phosphate(FW 137.99), disodium phosphate(FW 174.20), trisodium phosphate(FW 380.12) and MgCl2(FW 203.30)

a- Calculated mass of regeants needed to prepare 100ml of 275 mm solution of monobasic phosphate & 275 mM dibasic phosphate each containing 2.75 mm MgCl2.

Mono = 37.95g & Dibasic=47.91g

b- Calculate the volume of each of these solutions that will be combined to make 100 mL of a 275 mM sodium phosphate buffer containing 2.75 mM MgCl2 at pH 7.5

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