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How Much Cobalt is in Soil?

Discussion:

We agree that there was perfect positive correlation between the two variables, and is consistent with the Beer-Lambert Law and the current understanding of colorimetry which predicts that for a given light absorbing species, a plot of absorbance versus concentration should give a straight line (reference).In fact, There may have been a few discrepancies in the accuracy of the measurements taken during the experiment and this was because of the quality of the spectrometer and the difficulty of measuring out solutions.

Introduction:

The goal of this lab was to determine the connection between the absorbance and concentrations of cobalt in different solutions to estimate the concentration of cobalt nitrate in an unknown solution. Colorimetry was used in this lab to help determine concentration of cobalt nitrate solution from the soil. In addition, we have used the spectrometer light of a specific wavelength with different cobalt (II) nitrate concentrations ranging from 0.1M to 0.01M and a detector in the machine measured the light.

Procedures:

Firstly, calibrated the wavelength by putting distilled water into the chamber and calibrating it to where the absorbance reads 0.00. Then, replace the distilled water with the 0.1M solution of cobalt (II) and record the absorbance. Repeat these steps with the all wavelength. Secondly, by using a cylinder, measure out a minimum of 7 dilutions ranging from 0.1M all the way down to 0.01M. Thirdly, record all dilutions absorbance and calculate the absorbance of the unknown concentration sample cobalt (II) by using the colorimetry. Fourthly, use Beers-Lambert Law and calculate the unknown cobalt (II) concentration using the absorbance found earlier and also use the determined concentration of the unknown cobalt (II) solution and convert it to mg/kg.

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