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NMR (Nuclear Magnetic Resonance) steps:-->
N.B: NMR permits the study of proteins in solution and therefore does not require preparation of crystals.
N.B: In some cases NMR may represent fluctuations or " breathing " of the protein in solution. The similarity of the NMR and X-ray crystallography structures indicates that the protein structures found in crystals accurately represent the structure of the protein in solution.

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