Imagine you want to determine the binding constant for a reaction. You know you will need to generate data to calculate the binding constant. Summarize six logical steps to follow in order to determine experimentally the forward rate constant (k1) for the biomolecular binding reaction,
A+B AB. Hint - How would you set up the experiment to yield data values that enable determination of k1, and then how would you use that data to find k1? Show your work, including sketched graphs to illustrate the data trends.