Ask Question, Ask an Expert

+1-415-315-9853

info@mywordsolution.com

Ask Chemistry Expert

Compute the structural analysis of the Protein.

1. What order will the following proteins elute from a caution exchange column loaded at pH 6?
Protein A 10 kDa pI = 8.0
Protein B 15 kDa pI = 4.0
Protein C 25 kDa pI = 6.5
a. A, then B, then C
b. B, then C, then A
c. A, then C, then B
d. B, then A, then C

2. If the same proteins shown in problem 12 were run on gel filtration chromatography and SDS-PAGE, which of the following statements would be true?
a. Protein A would elute first using gel filtration chromatography and run fastest on SDS-PAGE.
b. Protein B would elute first using gel filtration chromatography and run fastest on SDS-PAGE.
c. Protein C would elute first using gel filtration chromatography and run fastest on SDS-PAGE.
d. Protein A would elute first using gel filtration chromatography while C would run fastest on SDS-PAGE.
e. Protein C would elute first using gel filtration chromatography while A would run fastest on SDS-PAGE.

3. Place the following steps for a round of Edman's degradation (sequencing) in order:
I. cleave the N-terminal amino acid
II. Label the N-terminal amino acid
III. Use HPLC to detect the N-terminal amino acid
a. First I, then II, then III, then repeat.
b. First II, then I, then III, then repeat.
c. First III, then II, then I, then repeat.
d. First I, then III, then II, then repeat.
e. First III, then I, then II, then repeat.

4. The formation of a peptide bond between two amino acids is an ex of a (n)______________ reaction.
a. cleavage
b. condensation
c. group transfer
d. isomerization
e. oxidation reduction

5. The average molecular weight of the 20 standard amino acids is 138, but biochemists use 110 when estimating the number of amino acids in a protein of known molecular weight. Why?
1. The number 110 is based on the fact that the average molecular weight of a protein is 110,000 with an average of 1,000 amino acids.
2. The number 110 reflects the higher proportion of small amino acids in proteins, as well as the loss of water when the peptide bond forms.
3. The number 110 reflects the number of amino acids found in the typical small protein, and only small proteins have their molecular weight estimated this way.
4. The number 110 takes into account the relatively small size of nonstandard amino acids.
5. The number 138 represents the molecular weight of conjugated amino acids.

6. Which of the following is correct with respect to the amino acid composition of proteins?
a. Larger proteins have a more uniform distribution of amino acids than smaller proteins.
b. Proteins contain at least one each of the 20 different standard amino acids.
c. Proteins with different functions usually differ significantly in their amino acid composition.
d. Proteins with the same molecular weight have the same amino acid composition.

7. The average molecular weight of an amino acid in a protein increases with the size of the protein. Which of the following refers to particularly stable arrangements of amino acid residues in a protein that give rise to recurring patterns?
a. Primary structure.
b. Secondary structure.
c. Tertiary structure.
d. Quaternary structure.
e. None of the above.

8. Which of the following describes the overall three-dimensional folding of a polypeptide?
a. Primary structure.
b. Secondary structure.
c. Tertiary structure.
d. Quaternary structure.
e. None of the above.

9. Which type of interaction stabilizes secondary structure?
1. van der Waals
2. hydrogen bonding
3. ionic (electrostatic)
4. covalent
5. all of the above

10. Which of the following best represents the backbone arrangement of two peptide bonds?
a. Cα-N-Cα-C-Cα-N-Cα-C
b. C α-C-N-Cα-C-N
c. C-N-Cα-Cα-C-N
d. Cα-N-C-C-N-Cα
e. Cα-Cα-C-N-Cα-Cα-C

Chemistry, Academics

  • Category:- Chemistry
  • Reference No.:- M919281

Have any Question? 


Related Questions in Chemistry

Consider a pipe with a length of 435 cm if the temperature

Consider a pipe with a length of 43.5 cm. If the temperature of the air is 19.5 oC and the pipe is closed in one end and open in the other, what is the frequency of the third harmonic for the pipe? Post your answer in he ...

Write the elemental symbol with charge for when aluminum

Write the elemental symbol, with charge, for when Aluminum forms an ion and tell whether it is an anion or a cation. Think about whether it gains or loses electrons.

Assignmentthe purpose of this assignmentsubmission of an

Assignment The Purpose of this Assignment Submission of an ePortfolio is required for this class. Your Signature Assignment should address topics of personal interest, societal concerns, local, or even global issues, fro ...

Assuming that air is 210 percent oxygen by volume what

Assuming that air is 21.0 percent oxygen by volume, what volume of air in liters at 45.0 °C and 793 mmHg would be required to burn 185 g of ethanol (C 2 H 5 OH)?

Potassium and sodium chloride are similar in charge and

Potassium and sodium chloride are similar in charge and they both have the same anion. When comparing sodium chloride (36 g / 100 mL at 0 oC) and potassium chloride, which has a higher solubility at 0 oC?

What is the sum of all coefficients when the following net

What is the sum of all coefficients when the following net ionic equation is balanced using the smallest whole number coefficients possible? Do not forget coefficients of one. MnO 4 - + Mn 2+ MnO 2

A sealed container holds 100 mole of helium and 200 moles

A sealed container holds 1.00 mole of helium and 2.00 moles of nitrogen at 30.0°C. When the total pressure of the mixture is 666 mm Hg, what is the partial pressure of the nitrogen in mm Hg? Provide an answer correct to ...

A 010 m fes04 aq solution is electrolyzed between a

A 0.10 M FeS04 (aq) solution is electrolyzed between a magnesium cathode and a platinum anode with a current density of 1.50 mA cm-2.The hydrogen over potential is 0.60 V. What will be the concentration of Fe2+ions when ...

If 628 g ca react with 923 g hcl according to the reaction

If 62.8 g Ca react with 92.3 g HCl according to the reaction below, how many grams of hydrogen gas will be produced, and how many grams of the excess reactant will be left over? Unbalanced equation: Ca + HCl → CaCl2+ H2 ...

What is the molarity of 200 l naoh solution prepared from

What is the molarity of 2.00 L NaOH Solution prepared from the dilution of 50.00 mL of 19.1 M NaOH?

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Section onea in an atwood machine suppose two objects of

SECTION ONE (a) In an Atwood Machine, suppose two objects of unequal mass are hung vertically over a frictionless

Part 1you work in hr for a company that operates a factory

Part 1: You work in HR for a company that operates a factory manufacturing fiberglass. There are several hundred empl

Details on advanced accounting paperthis paper is intended

DETAILS ON ADVANCED ACCOUNTING PAPER This paper is intended for students to apply the theoretical knowledge around ac

Create a provider database and related reports and queries

Create a provider database and related reports and queries to capture contact information for potential PC component pro

Describe what you learned about the impact of economic

Describe what you learned about the impact of economic, social, and demographic trends affecting the US labor environmen