Ask Question, Ask an Expert

+1-415-315-9853

info@mywordsolution.com

Ask Chemistry Expert

Compute the structural analysis of the Protein.

1. What order will the following proteins elute from a caution exchange column loaded at pH 6?
Protein A 10 kDa pI = 8.0
Protein B 15 kDa pI = 4.0
Protein C 25 kDa pI = 6.5
a. A, then B, then C
b. B, then C, then A
c. A, then C, then B
d. B, then A, then C

2. If the same proteins shown in problem 12 were run on gel filtration chromatography and SDS-PAGE, which of the following statements would be true?
a. Protein A would elute first using gel filtration chromatography and run fastest on SDS-PAGE.
b. Protein B would elute first using gel filtration chromatography and run fastest on SDS-PAGE.
c. Protein C would elute first using gel filtration chromatography and run fastest on SDS-PAGE.
d. Protein A would elute first using gel filtration chromatography while C would run fastest on SDS-PAGE.
e. Protein C would elute first using gel filtration chromatography while A would run fastest on SDS-PAGE.

3. Place the following steps for a round of Edman's degradation (sequencing) in order:
I. cleave the N-terminal amino acid
II. Label the N-terminal amino acid
III. Use HPLC to detect the N-terminal amino acid
a. First I, then II, then III, then repeat.
b. First II, then I, then III, then repeat.
c. First III, then II, then I, then repeat.
d. First I, then III, then II, then repeat.
e. First III, then I, then II, then repeat.

4. The formation of a peptide bond between two amino acids is an ex of a (n)______________ reaction.
a. cleavage
b. condensation
c. group transfer
d. isomerization
e. oxidation reduction

5. The average molecular weight of the 20 standard amino acids is 138, but biochemists use 110 when estimating the number of amino acids in a protein of known molecular weight. Why?
1. The number 110 is based on the fact that the average molecular weight of a protein is 110,000 with an average of 1,000 amino acids.
2. The number 110 reflects the higher proportion of small amino acids in proteins, as well as the loss of water when the peptide bond forms.
3. The number 110 reflects the number of amino acids found in the typical small protein, and only small proteins have their molecular weight estimated this way.
4. The number 110 takes into account the relatively small size of nonstandard amino acids.
5. The number 138 represents the molecular weight of conjugated amino acids.

6. Which of the following is correct with respect to the amino acid composition of proteins?
a. Larger proteins have a more uniform distribution of amino acids than smaller proteins.
b. Proteins contain at least one each of the 20 different standard amino acids.
c. Proteins with different functions usually differ significantly in their amino acid composition.
d. Proteins with the same molecular weight have the same amino acid composition.

7. The average molecular weight of an amino acid in a protein increases with the size of the protein. Which of the following refers to particularly stable arrangements of amino acid residues in a protein that give rise to recurring patterns?
a. Primary structure.
b. Secondary structure.
c. Tertiary structure.
d. Quaternary structure.
e. None of the above.

8. Which of the following describes the overall three-dimensional folding of a polypeptide?
a. Primary structure.
b. Secondary structure.
c. Tertiary structure.
d. Quaternary structure.
e. None of the above.

9. Which type of interaction stabilizes secondary structure?
1. van der Waals
2. hydrogen bonding
3. ionic (electrostatic)
4. covalent
5. all of the above

10. Which of the following best represents the backbone arrangement of two peptide bonds?
a. Cα-N-Cα-C-Cα-N-Cα-C
b. C α-C-N-Cα-C-N
c. C-N-Cα-Cα-C-N
d. Cα-N-C-C-N-Cα
e. Cα-Cα-C-N-Cα-Cα-C

Chemistry, Academics

  • Category:- Chemistry
  • Reference No.:- M919281

Have any Question? 


Related Questions in Chemistry

A sample of o2 was collected over water at 25c and 865 atm

A sample of O2 was collected over water at 25C and .865 atm. If the total sample volume was 2.538 L, how many moles of O2 were collected?

Consider a 065 m column with van deemter parameters a022 mm

Consider a 0.65 m column with van Deemter parameters A=0.22 mm, B=21.7 mm mL/min, and C=0.018 mm min/mL. If it takes at least 85 theoretical plates to fully separate two solutes, what range of flow rates can be used to s ...

Calculate the magnitude of the diffusion-controlled rate

Calculate the magnitude of the diffusion-controlled rate constant at 298 K for a species in (i) water, (ii) pentane. The viscosities are 1.00 × 10-3 kg m-1 s-1, and 2.2 ×10-4 kg m-1 s-1, respectively.

Write the balanced equation then outline the steps

Write the balanced equation, then outline the steps necessary to determine the information requested in each of the following a) the number of moles and the mass of Mg required to react with 5.00 g of HCl and produce MgC ...

A 400g sample of an ideal gas occupies 124l at 23 degrees

A 4.00g sample of an ideal gas occupies 1.24L at 23 degrees Celcius and 1.12bar. What will be its volume at 14 degrees Celcius and 1.50bar?

Write a reserch paper on gas chromatographythis is a 10

Write a reserch paper on Gas chromatography. This is a 10 pages research paper that should include an introduction on chromatography, principles of separation, type of columns, resolution, detection methods and applicati ...

Consider a pipe with a length of 435 cm if the temperature

Consider a pipe with a length of 43.5 cm. If the temperature of the air is 19.5 oC and the pipe is closed in one end and open in the other, what is the frequency of the third harmonic for the pipe? Post your answer in he ...

What volume of 384 m hydrochloric acid is required to

What volume of 3.84 M hydrochloric acid is required to consume 4.12 grams of zinc to form aqueous zinc chloride and hydrogen gas?

Assignmentpart 11the ph scale is generally considered as a

Assignment Part 1 1. The pH scale is generally considered as a scale from 1 to 14. State a value of pH considered to be: a. acidic b. basic c. neutral 2. The acid in our stomach is used to break down food proteins and ki ...

If you take a 100 ml portion of a 148 m ammonia solution

If you take a 10.0 mL portion of a 14.8 M ammonia solution and dilute it to a total volume of 0.250 L, what is the concentration of ammonia in the final solution? Provide an answer correct to 2 significant figures. For e ...

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Section onea in an atwood machine suppose two objects of

SECTION ONE (a) In an Atwood Machine, suppose two objects of unequal mass are hung vertically over a frictionless

Part 1you work in hr for a company that operates a factory

Part 1: You work in HR for a company that operates a factory manufacturing fiberglass. There are several hundred empl

Details on advanced accounting paperthis paper is intended

DETAILS ON ADVANCED ACCOUNTING PAPER This paper is intended for students to apply the theoretical knowledge around ac

Create a provider database and related reports and queries

Create a provider database and related reports and queries to capture contact information for potential PC component pro

Describe what you learned about the impact of economic

Describe what you learned about the impact of economic, social, and demographic trends affecting the US labor environmen