Ask Question, Ask an Expert

+1-415-315-9853

info@mywordsolution.com

Ask Chemistry Expert

Compute the structural analysis of the Protein.

1. What order will the following proteins elute from a caution exchange column loaded at pH 6?
Protein A 10 kDa pI = 8.0
Protein B 15 kDa pI = 4.0
Protein C 25 kDa pI = 6.5
a. A, then B, then C
b. B, then C, then A
c. A, then C, then B
d. B, then A, then C

2. If the same proteins shown in problem 12 were run on gel filtration chromatography and SDS-PAGE, which of the following statements would be true?
a. Protein A would elute first using gel filtration chromatography and run fastest on SDS-PAGE.
b. Protein B would elute first using gel filtration chromatography and run fastest on SDS-PAGE.
c. Protein C would elute first using gel filtration chromatography and run fastest on SDS-PAGE.
d. Protein A would elute first using gel filtration chromatography while C would run fastest on SDS-PAGE.
e. Protein C would elute first using gel filtration chromatography while A would run fastest on SDS-PAGE.

3. Place the following steps for a round of Edman's degradation (sequencing) in order:
I. cleave the N-terminal amino acid
II. Label the N-terminal amino acid
III. Use HPLC to detect the N-terminal amino acid
a. First I, then II, then III, then repeat.
b. First II, then I, then III, then repeat.
c. First III, then II, then I, then repeat.
d. First I, then III, then II, then repeat.
e. First III, then I, then II, then repeat.

4. The formation of a peptide bond between two amino acids is an ex of a (n)______________ reaction.
a. cleavage
b. condensation
c. group transfer
d. isomerization
e. oxidation reduction

5. The average molecular weight of the 20 standard amino acids is 138, but biochemists use 110 when estimating the number of amino acids in a protein of known molecular weight. Why?
1. The number 110 is based on the fact that the average molecular weight of a protein is 110,000 with an average of 1,000 amino acids.
2. The number 110 reflects the higher proportion of small amino acids in proteins, as well as the loss of water when the peptide bond forms.
3. The number 110 reflects the number of amino acids found in the typical small protein, and only small proteins have their molecular weight estimated this way.
4. The number 110 takes into account the relatively small size of nonstandard amino acids.
5. The number 138 represents the molecular weight of conjugated amino acids.

6. Which of the following is correct with respect to the amino acid composition of proteins?
a. Larger proteins have a more uniform distribution of amino acids than smaller proteins.
b. Proteins contain at least one each of the 20 different standard amino acids.
c. Proteins with different functions usually differ significantly in their amino acid composition.
d. Proteins with the same molecular weight have the same amino acid composition.

7. The average molecular weight of an amino acid in a protein increases with the size of the protein. Which of the following refers to particularly stable arrangements of amino acid residues in a protein that give rise to recurring patterns?
a. Primary structure.
b. Secondary structure.
c. Tertiary structure.
d. Quaternary structure.
e. None of the above.

8. Which of the following describes the overall three-dimensional folding of a polypeptide?
a. Primary structure.
b. Secondary structure.
c. Tertiary structure.
d. Quaternary structure.
e. None of the above.

9. Which type of interaction stabilizes secondary structure?
1. van der Waals
2. hydrogen bonding
3. ionic (electrostatic)
4. covalent
5. all of the above

10. Which of the following best represents the backbone arrangement of two peptide bonds?
a. Cα-N-Cα-C-Cα-N-Cα-C
b. C α-C-N-Cα-C-N
c. C-N-Cα-Cα-C-N
d. Cα-N-C-C-N-Cα
e. Cα-Cα-C-N-Cα-Cα-C

Chemistry, Academics

  • Category:- Chemistry
  • Reference No.:- M919281

Have any Question? 


Related Questions in Chemistry

The barrier to rotation about the c-c bond in bromoethane

The barrier to rotation about the C-C bond in bromoethane is 15kJ/mol (3.6kcal/mol). (a) What energy value can you assign to an H-Br eclipsing interaction? (b) Construct a quantitative diagram of potential energy versus ...

Instructions - show the calculation steps including all

Instructions - Show the calculation steps including ALL units and unit conversions. Present your work so that it is well organized. Problem 1 - A blended waste oil containing a sulfur content of 2.5%, a lead content of 0 ...

Aerogel or solid smoke is a novel material that is made of

Aerogel or "solid smoke" is a novel material that is made of silicon dioxide, like glass, but is a thousand times less dense than glass because it is extremely porous. Material scientists at NASA's Jet Propulsion Laborat ...

An ancient gold coin is 22 cm in diameter and 30 mm thick

An ancient gold coin is 2.2 cm in diameter and 3.0 mm thick. It is a cylinder for which volume = (p)(radius)2(thickness). If the density of gold is 19.3 g/cm3, what is the mass of the coin in grams?

Pre-lab1what is the equation for densitythe density

Pre-Lab: 1. What is the equation for density? The Density Calculator uses the formula that density (p) is equal to mass (M) divided by volume (V) or p=M/V. The calculator can use any two of the values to calculate the th ...

1 you want to make a solution of a solid sample for ir

1. You want to make a solution of a solid sample for IR analysis. Assuming that the solid dissolves in it, why would carbon tetrachloride be a good solvent to use? 2. How are UV/visible and IR spectrophotometry similar? ...

A sample of a hydrocarbon is found to contain 799g carbon

a sample of a hydrocarbon is found to contain 7.99g carbon and 2.01g hydrogen what is the empirical formula for this compound

Position paper - chem 121 6380 chemistry in the modern

Position Paper - CHEM 121 6380 Chemistry in the Modern World (2168) Position Paper: To Frack or not to Frack Imagine you are the administrator of the city or town where you currently reside. A rich deposit of shale oil h ...

A you have 50 ml of a buffer solution that is 015 m in ha

a. You have 50 mL of a buffer solution that is 0.15 M in HA and 0.25 M in A-. Calculate the pH of the solution after you add 100 mL of 0.01 M HCl to the solution. The pKa of HA is 4.75. b. You wish to prepare 100 mL of a ...

You are working with a chemical process in your factory

You are working with a chemical process in your factory with a Tubular reactor in ADIABATIC conditions. The conversion of compound A at the outlet of your reactor has a value of 50 %. The reaction mechanism of your react ...

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

A cola-dispensing machine is set to dispense 9 ounces of

A cola-dispensing machine is set to dispense 9 ounces of cola per cup, with a standard deviation of 1.0 ounce. The manuf

What is marketingbullwhat is marketing think back to your

What is Marketing? • "What is marketing"? Think back to your impressions before you started this class versus how you

Question -your client david smith runs a small it

QUESTION - Your client, David Smith runs a small IT consulting business specialising in computer software and techno

Inspection of a random sample of 22 aircraft showed that 15

Inspection of a random sample of 22 aircraft showed that 15 needed repairs to fix a wiring problem that might compromise

Effective hrmquestionhow can an effective hrm system help

Effective HRM Question How can an effective HRM system help facilitate the achievement of an organization's strate