1. How would you expect the amino acids glycine, alanine, and phenylalanine to travel relative to each other on the silica plates using a mixture of chloroform/methanol/ammonia? Explain your answer.
2.You spot a sample of an unknown pure compound on a TLC plate and elute it with hexane. It's Rf is 0.25. Would you expect its Rf to increase, decrease, or remain the same if you elute with acetone? Why?