Firefly luciferase is the enzyme that allows fireflies to illuminate their abdomens. Because this light generation is an ATP-requiring reaction, firefly luciferase can be used to test for the presence of ATP. In this way, luciferase can test for the presence of life. The coupled reactions are luciferin+O2ATPoxyluciferin+lightAMP+PPi
If the overall G of the coupled reaction is -4.50kJ/mol , what is the equilibrium constant, K, of the first reactions at 16?C ? The ?G for the hydrolysis of ATP to AMP is ?31.6