In normal living cells, the nitrogen requirement for the cells is provided from protein metabolism (i.e. consumption of the protein in the cells). When individual cells are commercially grown, (NH4)2SO4 is usually used as the source of nitrogen. Determine the amount of (NH4)2SO4 in g consumed in a fermentation medium in which the final cell concentration is 40 g/L in a 500 L medium volume. Assume that the cells contain 9% (by mass) nitrogen N, and that (NH4)2SO4 is the only nitrogen source. Mw for N is 14; Mw for (NH4)2SO4 is 132.