Determine the amino acid sequence of a heptapeptide from the following data. Briefly explain the meaning of the results in each of the following treatments.

a) Treatment with Edmanâ€^TMs reagent gives PTH-Ser.

b) Treatment with carboxypeptidase A releases Ala.

c) Treatment with cyanogen bromide yields two peptides. The amino acids contain of them are the followings:

1. Ala, Gly, Lys, Tyr

2. Met, Phe, Ser

d) Treatment with trypsin yields the following two peptides:

1. Lys, Met, Phe, Ser
2. Ala, Gly, Tyr

e) Treatment with chymotrypsin yields the following three peptides:

1. Lys, Met, Tyr
2. Ala, Gly
3. Phe, Ser

f) The sequence of this peptide is: