(Answered) Cotyledons experimenti need some help on the induction of

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Cotyledons Experiment

I need some help on the Induction of nitrate reductase by NO3- in radish seedling cotyledons:
20 pairs of cotyledons are incubated for 4 hours with different solutions,

Solution A: K2SO4, MgSO4, Ca(H2PO4)
Solution B: K2SO4, MgSO4, Ca(H2PO4), KNO3
Solution C: K2SO4, MgSO4, Ca(H2PO4), KNO3, cycloheximide
Solution D: K2SO4, MgSO4, Ca(H2PO4), KNO3, cycloheximide, chloramphenicol

The cotyledon extract was used to perform NR enzyme activity assay.
The following results were produced:

No incubation medium at zero time: 0.222 absorbance at 540nm
4hours incubation with-
Sol. A- 0.117 absorbance at 540nm
Sol B-0.209 absorbance at 540nm
Sol C-0.036 absorbance at 540nm
Sol D-0.362 absorbance at 540nm

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