1. Consider a chromatogram containing a single analyte with retention time of 4 minutes. The mobile phase elution time is 0.2 minutes. What is the capacity factor (k') for the analyte in question?
2. If 50 ?L of 0.03 molar analyte (from question 1) is injected into the column, how many moles of analyte are in the stationary phase during the separation?