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1) prepare in detail about different techniques used in cell number density determination

2) Compute the productivity (i.e., DP) of a chemostat under the following conditions:

i) Suppose Monod kinetics applies.

ii) Suppose the Luedeking – piret equation for product formation (qp=αµg+β) applies

D = 0.8µm                       YMX/S =0.5g X/g S            µm =1.0 h-1
S0 =1000 mg/L               α = 0.4 mg P/g X             β =0.5 h-1 mg P/g X
Ks =10 mg/L.

3) For an ideal chemostat, prove that: 1/YAPX/S = 1/YMX/S + ms/D.

4) Describe the unstructured model for microbial growth

5) Discuss about the bioreactor considerations for suspension culture of plant cells.

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