Compare the relative rate of the foward etoh as a substrate, Ask an Expert

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The values in the table are kinetic constants charactaristic of the foward reaction ( with ethanol + NAD+) and the back reaction (acetyldehyde +NADH) catalyzed by an ADH.

A. Compare the relative rate of the foward (EtOH as a substrate) and back reaction (with acetylaldehyde). Use the Michaelis-Menten equation to solve for v when ethanol is 99mM and acetylaldehyde is the substrate and its concentration is 1mM( This is early in the reaction; ethanol started at 3M/30 or 100mM).

Substrate: Km(mM) V max(rel)

EtOH 1.12 100

Acetylaldehyde 0.25 993

B. what do the kinetic constants tell you about the difficulty of getting a true Vo when measuring ADH activity with ethanol and NAD+?

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