Use Henderson-Hassalbach equation. The pKa of Tris-HCl is 8.1. After you have added 1 N HCl to adjust the pH of the Tris solution to 8.1, what is the ratio (in mmols) of Tris to Tris-HCl in the resulting buffer solution?

2.Based on the Henderson-Hassalbach equation how many mmols of Tris-HCl are there in a 100 mL of a 100 mM Tris-HCl buffer solution at pH 8.1? Note that the 100 mM refers to the sum of Tris and Tris-HCl concentrations. Answer in mmols

3.You now generate a NEW buffer solution by adding 0.1 mL of 10 M HCl to the solution in the previous question, how many mols of Tris-HCl would you now have in the NEW buffer solution? Answer in mmols.

4.What is the ratio of Tris-HCl to Tris (mmols) in the NEW buffer solution? Answer: __:1

5.Based on the Henderson-Hassalbach equation, what is the pH of the NEW buffer solution?