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You have isolated a unique mammalian protein, alpha, that recruits other partners in its normal function in the cell. Using a yeast two-hybrid system you have identified a potential binding partner, beta, for your bait protein.

Assuming that you could express and purify both components, describe TWO different experimental methodologies (including SALIENT details) that you would conduct to determine whether the two proteins interact in vivo in a mammalian cell line?

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