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Assume that the amino acids with ionizable side chains have the following pKas: Asp 3.65; Glu 4.25; His 6.0; Lys 10.53, Arg 12.48; Cys 8.18; Tyr 10.07. Also Assume that the a-amino group has a pKa of 9.62, and the a-carboxyl group has a pKa of 2.30. What would be the approximate pI value of the following protein (note that it is a hemoglobin, though that doesn't matter) --? (explain your logic)

Amino Acid - Moles/Mole Protein
Lys - 11
His - 6
Arg - 3
Asp - 8
Asn - 4
Thr - 1
Ser - 10
Glu - 4
Gln - 6
Pro - 3
Gly - 20
Ala - 28
Cys - 1
Val - 10
Met - 5
Ile - 8
Leu - 11
Tyr - 3
Phe - 3
Trp - 2

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