Analytical Methods:

Liquid chromatography (& NMR Practical/Demonstration)Safety Information

Paracetamol is classified as HARMFUL, IRRITATING to eyes, respiratory system and skin. Take care when handling and wear suitable protective clothing.

Methanol is flammable - Dispose of as directed

NMR - contains a strong magnetic field. Do not enter the room if you have a cardiac pacemaker or any ferromagnetic, electronic or mechanical device in your body. Serious injury may result. Magnetic fields may permanently damage watches, magnetic media and credit cards.

Cryogenic material (Liquid nitrogen) - is extremely cold; - do not touch dewar. Small amounts vaporise into nitrogen
Nitrogen- is an asphyxiant gas - if advised follow instructions to move to ventilated room.

In ALL laboratories you must:

Wear a fully fastened lab coat with sleeves down to wrists
Wear eye protection
Wash your hands when you leave

Please answer the following questions :

1. Development of an understanding of the fundamentals of HPLC and GC. Consideration of the underlying theory of separation processes.

2. Consideration of the theory and application of sample preparation for solid, liquid and air samples.

3. Prediction and interpretation of 1H and 13C NMR spectra of molecules of moderate complexity and to understand the basic instrumental aspects of the techniques.

4. Prediction and interpretation of the mass spectra of molecules of moderate complexity and to understand the basic instrumental aspects of the techniques.

5. Prediction and interpretation of IR spectra of molecules of moderate complexity and to understand the basic instrumental aspects of the techniques.

6. Prediction and interpretation of UV/Vis spectra of molecules of moderate complexity and to understand the basic instrumental aspects of the techniques

7.NMR Demo: Sample preparation and operation in NMR spectroscopy and/or an investigation into the sensitivity of NMR in comparison with other analytical techniques.

Academic requirements:

• Your work must be submitted as pages 7 of pages
• Student declaration must be completed.