An unknown solution contains only two absorbing species thymol blue and methylthymol blue. This sample had absorbance of 0.412 at 455 nm and 0.632 at 545 nm when measured using standard 1.000 cm cell, and the absorbance of blank at the same wavelengths as 0.003 and 0.005 respectively. The table below gives the molar absorptivities of the two molecules:
|
λ(nm)
|
ε thymol blue(1/Mcm)
|
ε mehylthymol blue(1/Mcm)
|
|
455 nm
|
4800
|
18900
|
|
545
|
36400
|
4450
|
Calculate the concentration of thymol blue and ethylthymol blue in the unknown sample.