An interesting enzyme 56 kda is used in catalytic amounts, Ask an Expert

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An interesting enzyme (56 kDa) is used in catalytic amounts (=very small) to cleave a membrane protein (65 kDa) into two fragments, 43 kDa and 22 kDa. Gel chromatography is used to assess the extent of the reaction at various times. Three major fractions of proteins are eluted at each time. What are they and in what order are they eluted?

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