An enzyme-catalyzed reaction produces a product that has a maximum absorbance at 412 nm. The extinction coefficient is 4000 M^-1 cm^-1. A 10-to-1 dilution of the enzyme is made and 20 microliter of the dilution are put into a cuvette with 80 microliter of water and 1.9 mL of reaction cocktail. The absorbance change per minute is 0.05.
(a) How many units are in the cuvette if 1 Units = 1 micromol/min?
(b) How many units are in the cuvette if 1 Units = 1 nanomol/min?
(c) What is the relative activity of the undiluted enzyme (1 Units = 1 micromol/min)?