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An enzyme-catalyzed reaction is performing in a 100ml solution containing 0.1M TRIS buffer. Explain the pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002mol of H+ were produced. Define what is the ratio of TRIS base to TRIS acid at the start of the experiment? Define what is the ratio at the end of the experiment? What is the final pH?

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