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An allosteric enzyme that follws the concerted mechanism (MWC model) has T/R ratio of 300 in the absence of substrate. Suppose that a mutation reversed the ratio. How would this mutation affect the relationship between the rate of the reaction (V) and substrate concentration ([S])? Select all that apply.

The enzyme would be less active

The enzyme would likely follow Michaelis-Menten kinetics

The plot of V vs. [S] would be shaped like an S, sigmoidal

The enzyme would be mostly in the R form

The plot of V vs. [S] would be shaped like a hyperbola

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